Fig. 2. Effect of Pra-B on TPA-induced cell migration and invasion in HeLa and SiHa cells. These cells were pretreated with TPA (50 ng/ml) for 2 h and then incubated with Pra-B (10 and 20 μM) for 24 h. Cell migration (A) and invasion (B) were examined using the Boyden chamber for 24 h (HeLa cells) and 18 h (SiHa cells) with polycarbonate filters, respectively. These results are representative of three independent experiments. The values shown are the mean ± SE. *P<0.01 compared with untreated cells. #P<0.01 compared with TPA alone.